Synthesis of Vaccinia Viral Proteins in Cytoplasmic Extracts
نویسندگان
چکیده
منابع مشابه
Synthesis of vaccinia viral proteins in cytoplasmic extracts. I. Incorporation of radioactively labeled amino acids into polypeptides.
Polypeptide synthesis has been studied in cell-free systems prepared from vaccinia virus-infected and uninfected HeLa cells. Cytoplasmic extracts containing endogenous messenger ribonucleic acid were used. Amino acid incorporation into hot trichloroacetic acid-precipitable material was linear for 15 to 20 min at 37 C. The initial rate of protein synthesis was approximately 15% of the rate in in...
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15 صفحه اولThe punctate sites of accumulation of vaccinia virus early proteins are precursors of sites of viral DNA synthesis.
Several vaccinia virus early proteins (encoded by genes B1R, H5R and I3L) synthesized in the presence of an inhibitor of DNA synthesis localize, at least in part, to punctate inclusions that are visible by immunofluorescence in the cytoplasm of poxvirus-infected cells. It is shown that these inclusions contain DNA (visualized by DAPI staining of the infected cells) and that the number of inclus...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1969
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.4.4.416-422.1969